Talk:Confocal laser scanning microscopy
|
|
Explained the image formation and the resolution enhancement as good as I understood it. However, I could not find the explanation of the resolution enhancement in the literature, i.e. I did not verify its correctness by consulting a specialist on this topic. I drew the figure of the confocal principle with Xfig.
To my knowledge, the enhancement of lateral resolution in CLSM is due to :
- Coherence of the laser light
- Small size of the illuminated field (point scanning)
see : "foundations of confocal scanned imaging in light microscopy" by Shinya Inoue in "Handbook of biological confoclal microscopy" JB Pawley,