Image:Blotautoradiogram.png
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Example of a Western blot using radioactivity. This is an image of a sheet of film that was used to detect radioactively labeled Protein A on a sheet of nitrocellulose. Portions of two different gels (upper and lower panels) were transferred to the nitrocellulose. One was reacted with a primary antibody specific for the cell-to-cell adhesion protein N-cadherin, the other with anti-E-cadherin antibody. The three lanes (0, 1, 2) contained cell proteins from different times (days) after exposure to a chemical inducer of cell differentiation. The results show that when the cells differentiated they shifted from expressing one cadherin to the other.
Source: my personal image.
Uploaded for the Western blot page.
The copyright to this image is retained by John Schmidt (JWSchmidt).
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